Journal: Journal of Cellular and Molecular Medicine

Article Title: MET inhibition enhances PARP inhibitor efficacy in castration‐resistant prostate cancer by suppressing the ATM/ATR and PI3K/AKT pathways

doi: 10.1111/jcmm.17037

Figure Lengend Snippet: γH2AX staining and ATM/ATR pathway downregulation following MET suppression and enhancing sensitivity to olaparib. (A) Representative immunofluorescent staining of γH2AX (red) and DAPI (blue) in DU145 and PC3 cells in the presence of Cri ((MET inhibitor crizotinib; 4 μM) and Ola (PARP inhibitor olaparib; 64 μM), Cri or Ola monotherapy, or dimethyl sulphoxide for 3 days. Scale bar, 50 μm. More than five foci per nucleus were considered as positive cells. (B) Western blot analysis of γH2AX expression in DU145 and PC3 cells. (C‐D) Western blot analysis of p‐ATM/ATM, p‐ATR/ATR, and RAD51 expression in DU145 and PC3 cells. Statistically significant differences were assessed by Student's t test in three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001. ns = no statistical difference

Article Snippet: Antibodies against GAPDH (6004–1‐Ig), BCL‐2 (60178–1‐Ig), ATM (27156–1‐AP), ATR (19787–1‐AP), IgG (H + L) (SA00013‐4), and RAD51 (14961–1‐AP) were purchased from Proteintech (Wuhan, China).

Techniques: Staining, Western Blot, Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: MET inhibition enhances PARP inhibitor efficacy in castration‐resistant prostate cancer by suppressing the ATM/ATR and PI3K/AKT pathways

doi: 10.1111/jcmm.17037

Figure Lengend Snippet: Olaparib and crizotinib synergistically inhibit the growth of subcutaneous tumours in vivo . Once DU145 subcutaneous tumour reached 50 mm 3 , mice were injected intraperitoneally with the olaparib (Ola, 40 mg/kg) and crizotinib (Cri, 5 mg/kg), either alone or in combination for 4 weeks (5 day per week). (A) Brief experiment process diagram. (B–D) Curves of tumour volume growth, representative gross images of tumour sizes, and tumour weight after treating with different groups as indicated. (E‐F) The representative immunohistochemical analysis of the expression of Ki67, RAD51, cleaved caspase 3, γH2AX, p‐PI3K, p‐AKT, p‐ATR and p‐ATM proteins in DU145 xenografted tumour cells after treating with different groups as indicated. Statistically significant differences were assessed by one‐way analysis of variance and Student's t test. * p < 0.05, ** p < 0.01, *** p < 0.001. ns = no statistical difference

Article Snippet: Antibodies against GAPDH (6004–1‐Ig), BCL‐2 (60178–1‐Ig), ATM (27156–1‐AP), ATR (19787–1‐AP), IgG (H + L) (SA00013‐4), and RAD51 (14961–1‐AP) were purchased from Proteintech (Wuhan, China).

Techniques: In Vivo, Injection, Immunohistochemical staining, Expressing

Journal: PLoS Genetics

Article Title: Elevated temperature increases meiotic crossover frequency via the interfering (Type I) pathway in Arabidopsis thaliana

doi: 10.1371/journal.pgen.1007384

Figure Lengend Snippet: (A, B) γH2Ax foci counting. (A) Pollen mother cells containing chromosomes (DAPI, blue) and γH2Ax foci (red). (B) Boxplots of γH2Ax foci at 20˚C and 28˚C; mean shown as white circle. (C, D) RAD51 foci counting. (C) Pollen mother cells containing chromosomes (DAPI, blue) and RAD51 foci (red). (D) Boxplots of RAD51 foci at 20°C and 28°C; mean shown as white circle. p-values are indicated as follows: ns = p > 0.5, * = p ≤ 0.05, ** = p ≤ 0.01. Scale bars represent 5 μm.

Article Snippet: The slides were incubated overnight with primary antibodies diluted 1:200 (RAD51, γH2AX, MLH1) in blocking buffer (goat serum, AR0009, Bosterbio) at 4°C and then at 37°C for 60 min with secondary antibody (1:1000, Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555, catalog # A-21428, Thermo Fisher Scientific).

Techniques: